| | |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| Protein Forum Protein Forum |
| | LinkBack | Thread Tools | Display Modes |
|
#1
| |||||||||||
| |||||||||||
| hi I prepared a flagella protein from bacteria. I want to add this now to cells and view it under the microscope. The idea is now that the flagella maybe bind to a specific location on the cell. However, to make this flagella protein viewable I have to stain it somehow with a (fluorescent) dye before I add it to the cells. How can I do that? Other possibilities are to clone a gfp-expessing flagella, which I already tried unsuccessfully. Or with help of a primary and secondary antibody, which is quite expensive. When I google I just find dyes for sds-gel stainings, like coomassie. Could this work for me as well? thanks for reading this! dominik |
| Tags |
| confocal , flagella staining , live , microscopy , protein , protein staining , staining |
| Thread Tools | |
| Display Modes | |
|
|
| | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| Human Cytome Project - Update 24 Jan. 2005 | Peter Van Osta | Cell Biology and Cell Culture | 1 | 08-01-2010 02:18 PM |
| Human Cytome Project - an idea - Update 19 April 2005 | Peter Van Osta | Cell Biology and Cell Culture | 1 | 06-01-2009 02:17 PM |
| Human Cytome Project - Update 6 Jan. 2005 | Peter Van Osta | Cell Biology and Cell Culture | 0 | 01-06-2005 10:18 AM |
| New Saccharomyces Sequences 11/27/04 | Mike Cherry | Yeast Forum | 0 | 11-28-2004 11:39 PM |
| New Saccharomyces Sequences 05/19/04 | SGD Sequences | Yeast Forum | 0 | 05-23-2004 04:06 PM |