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staining a protein for live confocal microscopy

staining a protein for live confocal microscopy - Protein Forum

staining a protein for live confocal microscopy - Protein Forum


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Old 11-19-2008, 02:51 PM
Pipette Filler
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Question staining a protein for live confocal microscopy



hi
I prepared a flagella protein from bacteria. I want to add this now to cells and view it under the microscope. The idea is now that the flagella maybe bind to a specific location on the cell.
However, to make this flagella protein viewable I have to stain it somehow with a (fluorescent) dye before I add it to the cells. How can I do that?

Other possibilities are to clone a gfp-expessing flagella, which I already tried unsuccessfully. Or with help of a primary and secondary antibody, which is quite expensive.

When I google I just find dyes for sds-gel stainings, like coomassie. Could this work for me as well?

thanks for reading this!
dominik
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confocal , flagella staining , live , microscopy , protein , protein staining , staining


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