on PAS for glycoprotein

Dear all,

I stained a gel with PAS(periodic acid stain) method for visualizing
glycoproteins.
However, even protein markers and the reconstructed protein expressed
by E. coli could be stained.
Whether the PAS method is specific for visualizing glycoproteins?
And how to explain the non-glycoprotein can also be stained?

I am appreciated of any help of you!

--
Cao Jianhao

Am 04.10.2008, 07:57 Uhr, schrieb jh <[Only registered users see links. ]>:


Many marker proteins are glycosylated, this is true for almost all
proteins that are secreted.

Since you did not say how you did the staining here a reference that I
know works:

Segrest,J.P. & Jackson,R.L.: Molecular Weight Determination of
Glycoproteins by Polyacrylamide Gel Electrophoresis in Sodium Dodecyl
Sulfate
Meth. Enzymol. 28 (1972) 54-63

G. Fairbanks, T.L. Steck & D.F.H. Wallach: Electrophoretic analysis of the
major polypeptides of the human erythrocyte membrane
Biochemistry 10 (1971) 2606-2617

Btw: It is much more convenient to do the PAS-staining on a western blot
than in the gel, the incubation times are shorter and less reagent is
required.

Thanks Dr Engelbert Buxbaum
I want to know how sensitive the PAS method is? If I want a
glycoprotein visulized on blot, how much sample should I load in the
gel?

2008/10/15 Dr Engelbert Buxbaum <[Only registered users see links. ]>:



--
Cao Jianhao

Department of Biochemistry
School of Lifesciences
Sun Yat-sen Uniersity
Xingang Road, west
Guangzhou, 510275
CHINA

Am 15.10.2008, 07:34 Uhr, schrieb jh <[Only registered users see links. ]>:


I do not have my old lab books at hand, but IIRC the sensitivity is
similar to CBB staining.

would you like to explain IIRC in more detail, for I have no idea about it?

2008/10/17 Dr Engelbert Buxbaum <[Only registered users see links. ]>:



--
Cao Jianhao

Department of Biochemistry
School of Lifesciences
Sun Yat-sen Uniersity
Xingang Road, west
Guangzhou, 510275
CHINA

In article <[Only registered users see links. ].net >, jh <[Only registered users see links. ]> wrote:

IIRC = If I Remember Correctly