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Calculations for specific activity

Calculations for specific activity - Protein Forum

Calculations for specific activity - Protein Forum


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Old 10-02-2008, 01:17 PM
Morales, Victor
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Default Calculations for specific activity



Lisa:

Specific activity (mCi/g) X molecular weight (g/mol) = mCi/mole ----->
200 mCi/g X 463.6 g/mol = 92720 mCi/mol

If you need it in mCi/mmole:

Specific activity (mCi/g) X molecular weight (g/mol) X 1 mol
__________________________________________________ ___________ =
mCi/mmole
1000 mmol

200 mCi/g X 463.6 g/mol X 1 mole
_____________________________________ = 92.72 mCi/mmole
1000 mmoles

Hope this helps,
Victor Morales

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Today's Topics:

1. Help with calculations for specific activity (Fasulo, Lisa M)


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Message: 1
Date: Tue, 30 Sep 2008 16:55:26 -0400
From: "Fasulo, Lisa M" <[Only registered users see links. ]>
Subject: [Protein-analysis] Help with calculations for specific
activity
To: <[Only registered users see links. ].indiana.edu>
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<3F4063B77CC1F445933078B93A0A0C720855F3CF@groamrex m05.amer.pfizer.com>
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I am trying to calculate the specific activity of my [3H]-ligand but
don't know how to do it. The package insert for my radioligand states
that the Specific Activity is 200 mCi/g but I need to know what the
Specific Activity is in Ci/mmol. How do I calculate this? The
molecular weight of the radioligand is 463.6 g/mol. Can anyone walk me
through the calculations to give the specific activity in Ci/mmol? The
only other info I have is the concentration of the radioligand which is
1mCi/mL.

Lisa



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