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Proteins Digest, Vol 41, Issue 1

Proteins Digest, Vol 41, Issue 1 - Protein Forum

Proteins Digest, Vol 41, Issue 1 - Protein Forum


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Old 10-02-2008, 05:56 AM
maria.holmberg@risoe.dk
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Default Proteins Digest, Vol 41, Issue 1



If I understand your question right it is just a question of
transferring mCi/g to mCi/mmol...
The equation states that the amount in moles is the amount in grams
divided by the molecular weight of the species (n=m/M).
Thus 200 mCi/g = 200 mCi/(1g/463.6 g/mol) = 200 mCi/(1/463.6)mol

Regards Maria

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Today's Topics:

1. Help with calculations for specific activity (Fasulo, Lisa M)


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Message: 1
Date: Tue, 30 Sep 2008 16:55:26 -0400
From: "Fasulo, Lisa M" <[Only registered users see links. ]>
Subject: [Protein-analysis] Help with calculations for specific
activity
To: <[Only registered users see links. ].indiana.edu>
Message-ID:

<3F4063B77CC1F445933078B93A0A0C720855F3CF@groamrex m05.amer.pfizer.com>
Content-Type: text/plain; charset="us-ascii"

I am trying to calculate the specific activity of my [3H]-ligand but
don't know how to do it. The package insert for my radioligand states
that the Specific Activity is 200 mCi/g but I need to know what the
Specific Activity is in Ci/mmol. How do I calculate this? The
molecular weight of the radioligand is 463.6 g/mol. Can anyone walk me
through the calculations to give the specific activity in Ci/mmol? The
only other info I have is the concentration of the radioligand which is
1mCi/mL.

Lisa



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