missing residues in pdb file

Hello,
I am having trouuble with missing residues in pdb files and need to
replace them (i.e. interpolate them) using a program. I think swiss-
pdbviewer and pymol do that. My problem is that I am not very capable
of using those programs and the person whom I asked for help told me
that I have to check for missing residues in pdb file, open swiss-
pdbviewer then add residue at correct place and finally she told me
that I might need to do an energy minimization and I am thinking that
I will definitely do a mistake there. I was wondering if anyone knows
a more automated way to get the missing residues inserted for me
because I am thinking that you can find missing residues in pdb and
and programs can read that info and add these missing residues
themselves automatically.. (but again maybe not..)

Thanks.

Sincerely,
Arif.

In article <[Only registered users see links. ]>, "ozgun.harmanci" <[Only registered users see links. ]> wrote:

Missing residues mean that they were too flexible in the crystal to be
detected by X-ray scattering. Which, crudely, means that they were
"everywhere". So just add the residues manually into some random
non-folded conformation avoiding close contacts with the rest of
the protein.

But keep in mind that in reality you don't know where these residues
really are => the new positions you built should not form a basis
for any analysis/results. In that respect, you will probably be better
off not building any missing residues into your PDB at all.

DK