Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protein Science > Protein Forum
Register Search Today's Posts Mark Forums Read

Protein Forum Protein Forum


Protocol needed from study the protein aggregation

Protocol needed from study the protein aggregation - Protein Forum

Protocol needed from study the protein aggregation - Protein Forum


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 04-24-2008, 03:42 AM
Niraikulam Ayyadurai
Guest
 
Posts: n/a
Default Protocol needed from study the protein aggregation




Hi all

Recently i got a mutant gene and showing higher solubility then wild type. we are assuming that our mutant preventing the aggregation of the protein.

I would like to study the thermal and chemical protein aggregation.

i would be happy If any one provide me detailed protocol to study this experiment.

expecting lot of reply from our group

thanks
__________________________________________________ _______________
Video: Get a glimpse of the latest in Cricket, Bollywood, News and Fashion.Only on MSN videos.
[Only registered users see links. ]
Reply With Quote
  #2  
Old 04-28-2008, 07:28 PM
Dr Engelbert Buxbaum
Guest
 
Posts: n/a
Default Protocol needed from study the protein aggregation

Am 23.04.2008, 23:42 Uhr, schrieb Niraikulam Ayyadurai
<[Only registered users see links. ]>:


Differential scanning calorimetry would be my method of choice. No point
in posting the protocol, you need to find somebody with the required
equiment to collaborate with. Plan on sending them plenty of protein
(several 100 mg), there is a wide gap between what biophysicists and
biochemists consider "a small sample". And biophysicists don't do protein
purificantion and hence have absolutely no respect for the work you put in
;-(
Reply With Quote
Reply

Tags
aggregation , needed , protein , protocol , study


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Human Cytome Project - Update 24 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 1 08-01-2010 02:18 PM
Human Cytome Project - an idea - Update 19 April 2005 Peter Van Osta Cell Biology and Cell Culture 1 06-01-2009 02:17 PM
Human Cytome Project - Update 6 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 0 01-06-2005 11:18 AM
New Saccharomyces Sequences 11/27/04 Mike Cherry Yeast Forum 0 11-29-2004 12:39 AM
New Saccharomyces Sequences 05/19/04 SGD Sequences Yeast Forum 0 05-23-2004 04:06 PM


All times are GMT. The time now is 06:35 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.16999 seconds with 16 queries