Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protein Science > Protein Forum
Register Search Today's Posts Mark Forums Read

Protein Forum Protein Forum


Determination of oxytocin in biological samples by isocratichigh-performance liquid chromatography with coulometric detection using C18solid-phase extraction and polyclonal antibody-based immunoaffinity columnpurification

Determination of oxytocin in biological samples by isocratichigh-performance liquid chromatography with coulometric detection using C18solid-phase extraction and polyclonal antibody-based immunoaffinity columnpurification - Protein Forum

Determination of oxytocin in biological samples by isocratichigh-performance liquid chromatography with coulometric detection using C18solid-phase extraction and polyclonal antibody-based immunoaffinity columnpurification - Protein Forum


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 03-21-2008, 07:38 PM
tocopherol
Guest
 
Posts: n/a
Default Determination of oxytocin in biological samples by isocratichigh-performance liquid chromatography with coulometric detection using C18solid-phase extraction and polyclonal antibody-based immunoaffinity columnpurification



Determination of oxytocin in biological samples by isocratic high-
performance liquid chromatography with coulometric detection using C18
solid-phase extraction and polyclonal antibody-based immunoaffinity
column purification. J Chromatogr B Biomed Appl. 1994 Mar 4;653(2):
139-45.

Kukucka Mark A. and Misra, Hara P.

Department of Biomedical Sciences, Virginia-Maryland Regional College
of Veterinary Medicine, Virginia Polytechnic Institute and State
University, Blacksburg 24061-0442.

ABSTARCT

A specific high-performance liquid chromatographic (HPLC) method is
described for the reliable quantitation of oxytocin using culture
media supernatants. The procedure employs solid-phase extraction,
antibody-based immunoaffinity purification and isocratic HPLC with
dual channel coulometric detection (ED). The lower limit of detection
for this cyclic nonapeptide was 40 pg (40 fmol). Due to its relative
simplicity, specificity and precision, the HPLC-ED of oxytocin is an
accurate and attractive alternative to many existing quantitative
methods.
Reply With Quote
Reply

Tags
antibodybased , biological , c18solidphase , chromatography , columnpurification , coulometric , detection , determination , extraction , immunoaffinity , isocratichighperformance , liquid , oxytocin , polyclonal , samples


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Determination of oxytocin in biological samples by isocratichigh-performance liquid chromatography with coulometric detection using C18solid-phase extraction and polyclonal antibody-based immunoaffinity columnpurification tocopherol Forum Chemie 1 10-10-2012 02:11 PM
Determination of oxytocin in biological samples by isocratichigh-performance liquid chromatography with coulometric detection using C18solid-phase extraction and polyclonal antibody-based immunoaffinity columnpurification tocopherol Protocols and Methods Forum 0 11-29-2008 04:24 PM
Determination of oxytocin in biological samples by isocratichigh-performance liquid chromatography with coulometric detection using C18solid-phase extraction and polyclonal antibody-based immunoaffinity columnpurification tocopherol Cell Biology and Cell Culture 0 11-29-2008 04:00 PM


All times are GMT. The time now is 03:07 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.10963 seconds with 16 queries