Determination of oxytocin in biological samples by isocratichigh-performance liquid chromatography with coulometric detection using C18solid-phase extraction and polyclonal antibody-based immunoaffinity columnpurification

Determination of oxytocin in biological samples by isocratic high-
performance liquid chromatography with coulometric detection using C18
solid-phase extraction and polyclonal antibody-based immunoaffinity
column purification. J Chromatogr B Biomed Appl. 1994 Mar 4;653(2):
139-45.

Kukucka Mark A. and Misra, Hara P.

Department of Biomedical Sciences, Virginia-Maryland Regional College
of Veterinary Medicine, Virginia Polytechnic Institute and State
University, Blacksburg 24061-0442.

ABSTARCT

A specific high-performance liquid chromatographic (HPLC) method is
described for the reliable quantitation of oxytocin using culture
media supernatants. The procedure employs solid-phase extraction,
antibody-based immunoaffinity purification and isocratic HPLC with
dual channel coulometric detection (ED). The lower limit of detection
for this cyclic nonapeptide was 40 pg (40 fmol). Due to its relative
simplicity, specificity and precision, the HPLC-ED of oxytocin is an
accurate and attractive alternative to many existing quantitative
methods.