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Proteins Digest, Vol 32, Issue 11

Proteins Digest, Vol 32, Issue 11 - Protein Forum

Proteins Digest, Vol 32, Issue 11 - Protein Forum


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Old 01-21-2008, 01:54 AM
Clement Angkawidjaja
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Default Proteins Digest, Vol 32, Issue 11



I purified a nuclear receptor once and it requires high concentration of
salt (>500 mM) to remain soluble. Other osmolytes (glycerol, non-ionic
detergents) can replace salt for nuclear receptors (confirmed). I also heard
(although have not tried it) that macromolecular crowding agents (PEG20K,
ficol70) can also increase solubility. The point is, many intracellular
proteins, especially DNA-interacting ones stay stable in a "crowded"
environment (the cytosol and nucleoplasma are crowded).

The selection of crowding agents will depend on what experiments you will do
with your purified proteins.

Clement


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