We are using 13% Resolving and 4% stacking gel and tris glycine SDS
electrohoresis buffer with discontinuous gel system. We had tried out the
silver staining technique mentioned in the EP / USP 30 pharmacopoeia since
we have to comply pharmacopoeia requirements for our protein. But the gel
darkens and method is not very sensitive. Is there something wrong in the
procedure we are following. Can anyone tell me exact procedure to perform
silver stg as per USP and precautions to be taken.