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Problems with silver staining

Problems with silver staining - Protein Forum

Problems with silver staining - Protein Forum


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  #1  
Old 10-29-2007, 09:15 AM
terence diane fabella
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Default Problems with silver staining



Hi Mrs Dima Klenchin,

I have read your online conversation with Matt Parker regarding his problem with Biorad silver stain plus kit. You mentioned in your conversation, that you could give Matt a copy of the protocol on how to make a silver stain, if he likes. Can I ask a copy of it too? I am curently having my thesis and Coomasie stain is not staining my target protein I will try to use silver stain then. I will be very grateful if you could give me a copy of it. Thank you very much.

Terence

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  #2  
Old 10-30-2007, 07:11 PM
Dr Engelbert Buxbaum
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Default Problems with silver staining

Am 29.10.2007, 05:15 Uhr, schrieb terence diane fabella
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A good method is described in

@article{Heu-88,
AUTHOR= {J. Heukeshoven and R. Dernick},
TITLE= {Improved Silver Staining Procedure for fast Staining in
PhastSystem Development Unit I. Staining of Sodium Dodecyl Sulfate Gels},
JOURNAL= {Electrophoresis},
VOLUME= {9},
YEAR= {1988},
PAGES= {28-32},
LANGUAGE= {engl}
}

Instead of the capricious silver stain, you may also want to look at

@article{Rab-01,
AUTHOR= {T. Rabilloud and J.-M. Strub and S. Luche and A. van
Dorsselaer and J. Lunardi},
TITLE= {A comparison between Sypro Ruby and ruthenium(II) tris
(bathophenanthroline disulfonate) as fluorescent stains for protein
detection in gels},
JOURNAL= {Proteomics},
VOLUME= {1},
YEAR= {2001},
PAGES= {699-704},
LANGUAGE= {engl}
}

@article{Plu-07,
AUTHOR= {F. Pluder and A.G. Beck-Sickinger},
TITLE= {One-step procedure for staining of proteins with ruthenium
II tris (bathophenanthroline disulfonate)},
JOURNAL= {Anal. Biochem.},
VOLUME= {361},
YEAR= {2007},
PAGES= {299-301},
LANGUAGE= {engl}

And there are of course also the proprietary Sypro-stains originally
marketed by Molecular Probes (now Invitrogen).
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  #3  
Old 01-16-2008, 06:04 PM
Dr Engelbert Buxbaum
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Default Problems with silver staining

Am 29.10.2007, 05:15 Uhr, schrieb terence diane fabella
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Heukeshoven & Dernick: Electrophoresis 9 (1988) 28-32 works well. You do
not need the different temperatures, just working at 37 deg C for all
steps is fine.
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