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silver stained band at the running front

silver stained band at the running front - Protein Forum

silver stained band at the running front - Protein Forum


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Old 10-22-2007, 09:59 PM
Monica Fazzini
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Default silver stained band at the running front



Hi,
I've been running some of my samples on 4-20% SDS_PAGE, and I've noticed that when I silver stain, a band appeard at the running front. This band doesn't appear in lane without sample just loaded with Loading buffer. In addition, it stains more greyish than the rest of the proteins that are more brownish. Any suggestion about the nature of the band?
Thank you,
Monica
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Old 02-21-2012, 06:23 AM
Pipette Filler
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Default Re: silver stained band at the running front

Hi,
i just want a standard protocol for silver staining of sds gel.

Thanks in advance
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