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[Protein-analysis] Re: Proteins Digest, Vol 29, Issue 1

[Protein-analysis] Re: Proteins Digest, Vol 29, Issue 1 - Protein Forum

[Protein-analysis] Re: Proteins Digest, Vol 29, Issue 1 - Protein Forum


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Old 10-04-2007, 08:35 AM
Rhiannon Evans
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Default [Protein-analysis] Re: Proteins Digest, Vol 29, Issue 1



Hi Sai,

we use the stratagene quick change kit in our lab, it works a treat
providing you have the correct conditions for your PCR in the first
place.
I often find the best way to figure this out is to do a temperature
gradient, a Mg gradient and a DMSO gradient. Although there is MgSO4
in the buffer i still needed to add an extra 2 mM to my samples. DMSO
helps with preventing the formation of secondary structures also.

Good luck!

Rhiannon
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On 2 Oct 2007, at 21:39, Sai Praveen wrote:


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Old 10-05-2007, 04:31 AM
DK
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Default [Protein-analysis] Re: Proteins Digest, Vol 29, Issue 1

Rhiannon Evans <[Only registered users see links. ].uk> wrote:

Just out of curiousity: Why do you buy this overpriced kit when all
it contains is polymerase, dNTPs, DpnI, DMSO and competent
cells - all of which you are very likely to have already (and at 2-10X
less price)?

DK
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