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-   -   [Protein-analysis] Re: Proteins Digest, Vol 29, Issue 1 (http://www.molecularstation.com/forum/protein-forum/26954-%5Bprotein-analysis%5D-re-proteins-digest-vol-29-issue-1-a.html)

Rhiannon Evans 10-04-2007 08:35 AM

[Protein-analysis] Re: Proteins Digest, Vol 29, Issue 1
 
Hi Sai,

we use the stratagene quick change kit in our lab, it works a treat
providing you have the correct conditions for your PCR in the first
place.
I often find the best way to figure this out is to do a temperature
gradient, a Mg gradient and a DMSO gradient. Although there is MgSO4
in the buffer i still needed to add an extra 2 mM to my samples. DMSO
helps with preventing the formation of secondary structures also.

Good luck!

Rhiannon
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RKA Group
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Cardiff University
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On 2 Oct 2007, at 21:39, Sai Praveen wrote:



DK 10-05-2007 04:31 AM

[Protein-analysis] Re: Proteins Digest, Vol 29, Issue 1
 
Rhiannon Evans <[Only registered and activated users can see links. Click Here To Register...].uk> wrote:

Just out of curiousity: Why do you buy this overpriced kit when all
it contains is polymerase, dNTPs, DpnI, DMSO and competent
cells - all of which you are very likely to have already (and at 2-10X
less price)?

DK


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