[Protein-analysis] Re: Proteins Digest, Vol 29, Issue 1
 

Hi Sai,

we use the stratagene quick change kit in our lab, it works a treat
providing you have the correct conditions for your PCR in the first
place.
I often find the best way to figure this out is to do a temperature
gradient, a Mg gradient and a DMSO gradient. Although there is MgSO4
in the buffer i still needed to add an extra 2 mM to my samples. DMSO
helps with preventing the formation of secondary structures also.

Good luck!

Rhiannon
************************************************
Rhiannon Mari Evans
RKA Group
School of Chemistry
Cardiff University
Main Building
Park Place
Cardiff
CF10 3AT

Mobile: 07828 156417
Office/Lab : 029 20 879417
Email: [Only registered and activated users can see links. Click Here To Register...]

************************************************





On 2 Oct 2007, at 21:39, Sai Praveen wrote:

[Protein-analysis] Re: Proteins Digest, Vol 29, Issue 1
 

Rhiannon Evans <[Only registered and activated users can see links. Click Here To Register...].uk> wrote:

Just out of curiousity: Why do you buy this overpriced kit when all
it contains is polymerase, dNTPs, DpnI, DMSO and competent
cells - all of which you are very likely to have already (and at 2-10X
less price)?

DK