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[Protein-analysis] Re: Proteins Digest, Vol 29, Issue 1

[Protein-analysis] Re: Proteins Digest, Vol 29, Issue 1 - Protein Forum

[Protein-analysis] Re: Proteins Digest, Vol 29, Issue 1 - Protein Forum


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Old 10-03-2007, 08:56 PM
jkellosa@mappi.helsinki.fi
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Default [Protein-analysis] Re: Proteins Digest, Vol 29, Issue 1




Hey Sai,

In the lab where I worked for my previous project the people used only the
Stragenes Quick-Change kit and I also used it to make dozens of different
mutations in a few different proteins. With them and with me it worked 98-
95 percent of the time, respectively.

If you want to check for the possibility of secondary structure formation
in your primers, you can use the netprimer applet or download a free oligo-
analysis program from oligotech (you can find both of these if you just
type netprimer or oligotech in to google)

Cheers,

-Juho


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