Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum

Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum (http://www.molecularstation.com/forum/)
-   Protein Forum (http://www.molecularstation.com/forum/protein-forum/)
-   -   [Protein-analysis] Re: Proteins Digest, Vol 29, Issue 1 (http://www.molecularstation.com/forum/protein-forum/26953-%5Bprotein-analysis%5D-re-proteins-digest-vol-29-issue-1-a.html)

jkellosa@mappi.helsinki.fi 10-03-2007 08:56 PM

[Protein-analysis] Re: Proteins Digest, Vol 29, Issue 1
 

Hey Sai,

In the lab where I worked for my previous project the people used only the
Stragenes Quick-Change kit and I also used it to make dozens of different
mutations in a few different proteins. With them and with me it worked 98-
95 percent of the time, respectively.

If you want to check for the possibility of secondary structure formation
in your primers, you can use the netprimer applet or download a free oligo-
analysis program from oligotech (you can find both of these if you just
type netprimer or oligotech in to google)

Cheers,

-Juho




All times are GMT. The time now is 05:18 AM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved

Page generated in 0.08806 seconds with 11 queries