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Proteins Digest, Vol 26, Issue 1

Proteins Digest, Vol 26, Issue 1 - Protein Forum

Proteins Digest, Vol 26, Issue 1 - Protein Forum


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Old 07-02-2007, 04:03 PM
Sandeep
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Default Proteins Digest, Vol 26, Issue 1



Hello All

I am a new member in here, Could anyone guide me if we have a protein
database where I can look for physicochemical properties like solubility,
pI, structure , molecular weight for different proteins.

Kind Regards
Sandeep Yadav
Graduate Student
Department of Pharmaceutical Sciences,
School Of Pharmacy, University of Connecticut
69 North Eagleville Road, Unit 3092
Storrs, CT, 06269
Ph: 8604864754 (W)

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Today's Topics:

1. Bizzare Posttranslational modification during E. coli
overexpression (nomadiq)


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Message: 1
Date: Fri, 29 Jun 2007 18:35:01 -0000
From: nomadiq <[Only registered users see links. ]>
Subject: [Protein-analysis] Bizzare Posttranslational modification
during E. coli overexpression
To: [Only registered users see links. ]
Message-ID: <1183142101.145584.15580@e16g2000pri.googlegroups. com>
Content-Type: text/plain; charset="iso-8859-1"

Hi,

Im pretty sure a protein I am overexpressing from a pET15B vector has
a bizzare posttranslational modification. Mass spec has identified the
added mass as 958 Da (to about 20% of the protein molecules). Im
overexpressing in BL21 Lambda DE3 and my protein is cleaved away from
the His tag with thrombin.

Anyone have any ideas what kind of modification would add 958 Da? I
have checked and it doesn't seem the mass can be explained by added
amino acids.

Thanx in advance,

S.



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