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Quantitation of the octapeptide oxytocin using HPLC-ECD and Ab column purification

Quantitation of the octapeptide oxytocin using HPLC-ECD and Ab column purification - Protein Forum

Quantitation of the octapeptide oxytocin using HPLC-ECD and Ab column purification - Protein Forum


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Old 06-21-2007, 05:50 PM
tocopherol
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Default Quantitation of the octapeptide oxytocin using HPLC-ECD and Ab column purification



Isocratic High Performance Liquid Chromatography - Coulometric
Electrochemical Detection of Oxytocin in Biological Samples Using C18
Solid Phase Extraction and Polyclonal Antibody-Based Immunoaffinity
Column Purification

by Dr. Mark A. Kukucka and Dr. Hara P. Misra

Department of Biomedical Sciences
Virginia-Maryland Regional College of Veterinary Medicine
Virginia Polytechnic Institute & State University
Blacksburg, Virginia 24061-0442

Published in: Journal of Chromatography - Biomedical Applications


ABSTRACT: A rapid, sensitive and very specific high-performance liquid
chromatographic (HPLC) method is described for the reliable and
selective quantitation of oxytocin using culture media supernatants.
The isocractic procedure employs solid phase extraction, antibody-
based immunoaffinity purification and dual channel coulometric
electrochemical detection (ECD). The lower limit of detection for this
octapeptide was 40 picograms (40 fmol) on column. Due to its relative
simplicity, specificity and precision, the HPLC-ECD of oxytocin is an
accurate and attractive alternative to many existing quantitative
methods.


Running Title: HPLC-ECD of Oxytocin Using an Ab Column

Key Words: Oxytocin, HPLC, ECD, SPE, Antibody column


Kukucka, Mark A. and Hara P. Misra. 1994. Determination of oxytocin
in biological samples by isocratic high-performance liquid
chromatography with coulometric detection using C18 solid-phase
extraction and polyclonal antibody-based immunoaffinity column
purification. J Chromatogr B Biomed Appl 653(2):139-145.

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column , hplcecd , octapeptide , oxytocin , purification , quantitation


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