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Transcription Factor Binding Sites and DNA-Direction

Transcription Factor Binding Sites and DNA-Direction - Protein Forum

Transcription Factor Binding Sites and DNA-Direction - Protein Forum


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Old 04-05-2007, 11:43 AM
ALiX
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Default Transcription Factor Binding Sites and DNA-Direction



Hi,

Concerning transcription factors (TFs) and their binding sites and
motifs: does anyone know whether TFs can bind to their DNA-sequence
motifs in both directions? For example if a TF binds to a motif such
as 5'-GGGTTT-3', will it also bind to its reverse 5'-TTTGGG-3'? I'm
specifically interested in experiments done in vitro where the
cellular machinery does not get in the way (I'm looking at results
from EMSA with synthetic DNA-sequences).

Thanks,
/ALiX

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binding , dnadirection , factor , sites , transcription


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