Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protein Science > Protein Forum
Register Search Today's Posts Mark Forums Read

Protein Forum Protein Forum


Mechanism Of Cold Denaturation

Mechanism Of Cold Denaturation - Protein Forum

Mechanism Of Cold Denaturation - Protein Forum


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 01-15-2007, 01:25 PM
Protenger
Guest
 
Posts: n/a
Default Mechanism Of Cold Denaturation



Hi all
I'm confused about cold denaturation, I read "cold denaturation of
proteins" by privalov, but beside
thermodynamics explanation of this phenomenon there must be molecular
mechanism by which this type of denaturation can be described.
I would be appreciated if anybody give simple description of
thermodynamics concept of this event
but also molecular mechanism of that.
sorry for bad english

Thanks for your help
Protenger

Reply With Quote
  #2  
Old 01-15-2007, 05:06 PM
Dr Engelbert Buxbaum
Guest
 
Posts: n/a
Default Mechanism Of Cold Denaturation

Protenger wrote:



The bond energies of the various interactions that are responsible for
secondary and tertiary structure are energy dependent, as is the motion
of the atoms that make up proteins. The temperature coefficients however
are different, so that bonds become dominating at one temperature, which
are not dominating at another. As a result in the cold the structure of
lowest free energy may be different from that at 37 degrees. Especially
if you cool slowly, thus allowing the new structure to be achieved
(anealing). Cooling quickly may trap the protein in the higher
temperature structure, since the mobility drops with temperature. That's
why we snap-freeze our samples.


Reply With Quote
  #3  
Old 01-16-2007, 10:30 AM
Protenger
Guest
 
Posts: n/a
Default Mechanism Of Cold Denaturation

Dr Engelbert Buxbaum wrote:


Thanks for your help, but what is the reason of weakened hydrophobic
interactions at low temperature (in spite of lower entropy), and what
is the role of whater molecules in destabilizing native structure
( indeed destabilizing hydrophobic interactions) of proteins in cold
denaturation event?
Thank You

Reply With Quote
  #4  
Old 01-16-2007, 10:47 AM
Protenger
Guest
 
Posts: n/a
Default Mechanism Of Cold Denaturation


Protenger wrote:
Thanks for your help, but what is the reason of weakened hydrophobic
interactions at low temperature (in spite of lower entropy), and what
is the role of whater molecules in destabilizing native structure
( indeed destabilizing hydrophobic interactions) of proteins in cold
denaturation event?
Thank You

Reply With Quote
  #5  
Old 01-16-2007, 10:54 AM
Protenger
Guest
 
Posts: n/a
Default Mechanism Of Cold Denaturation


Dr Engelbert Buxbaum wrote:

Thanks for your help, but what is the reason of weakened hydrophobic
interactions at low temperature (in spite of lower entropy), and what
is the role of whater molecules in destabilizing native structure
( indeed destabilizing hydrophobic interactions) of proteins in cold
denaturation event?
Thank You

Reply With Quote
  #6  
Old 01-16-2007, 10:55 AM
Protenger
Guest
 
Posts: n/a
Default Mechanism Of Cold Denaturation


Dr Engelbert Buxbaum wrote:

Thanks for your help, but what is the reason of weakened hydrophobic
interactions at low temperature (in spite of lower entropy), and what
is the role of water molecules in destabilizing native structure
( indeed destabilizing hydrophobic interactions) of proteins in cold
denaturation event?
Thank You

Reply With Quote
Reply

Tags
cold , denaturation , mechanism


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
How do cold weather plants survive freezing? Alan Meyer Botany Forum 7 01-31-2007 01:50 AM
Conferenza Internazionale sulle Nuove Energie wortex Forum Chimica 11 02-21-2005 03:18 PM
Mechanism of sensing Wave Functions Consc Physics Forum 2 12-28-2004 08:33 PM
Sales Motivation & Cold Calling Seminar - only 97 pounds George Millar Protocols and Methods Forum 0 09-28-2003 11:19 PM
Sales Motivation & Cold Calling Seminar - only 97 pounds Paul Worthington Microbiology Forum 0 09-23-2003 10:36 AM


All times are GMT. The time now is 05:54 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.13956 seconds with 16 queries