Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protein Science > Protein Forum
Register Search Today's Posts Mark Forums Read

Protein Forum Protein Forum


Protein purification using mouse anti-Myc Tag IgG

Protein purification using mouse anti-Myc Tag IgG - Protein Forum

Protein purification using mouse anti-Myc Tag IgG - Protein Forum


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 12-21-2006, 05:19 PM
Breslauer
Guest
 
Posts: n/a
Default Protein purification using mouse anti-Myc Tag IgG




Hi there,

have question about publications & your experience. I'm going to purify
partially purifed recombinant protein (but containing ~30% of protein
contaminations) using affinity chromatography. I produced a lot of
monoclonal antibody - mouse anti-myc, and my protein has Myc-Tag.
Saying produced I mean collected supernatant from hybrydoma cells. The
thing is to purify & bind my antibodies and (it would be great if using
the same resign/column) pass my protein throuh it, hoping it will bind
with antibodies. Then wash & elute it. Do u thing that sepharose with
protein A will be good? Does any of u who have expereince in this
experiment may discuss this idea of expriment with me? Waiting for your
replay.

Bres

Reply With Quote
Reply

Tags
antimyc , igg , mouse , protein , purification , tag


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Human Cytome Project - Update 24 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 1 08-01-2010 02:18 PM
Human Cytome Project - an idea - Update 19 April 2005 Peter Van Osta Cell Biology and Cell Culture 1 06-01-2009 02:17 PM
Human Cytome Project - Update 6 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 0 01-06-2005 11:18 AM
New Saccharomyces Sequences 11/27/04 Mike Cherry Yeast Forum 0 11-29-2004 12:39 AM
New Saccharomyces Sequences 05/19/04 SGD Sequences Yeast Forum 0 05-23-2004 04:06 PM


All times are GMT. The time now is 02:01 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.16169 seconds with 16 queries