Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protein Science > Protein Forum
Register Search Today's Posts Mark Forums Read

Protein Forum Protein Forum


Western blot on Coomassie stained SDS-PAGE gel?

Western blot on Coomassie stained SDS-PAGE gel? - Protein Forum

Western blot on Coomassie stained SDS-PAGE gel? - Protein Forum


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 11-28-2006, 01:38 PM
kaydamov
Guest
 
Posts: n/a
Default Western blot on Coomassie stained SDS-PAGE gel?



Dear Andreas Savelsbergh,

I was founding in an old forum, that you routinely use Coomassie stained
gels directly for electroblotting and subsequent antibody detection. I also
used Coomassie stained gels but until now only for immunoblotting on
PVDF-membranes. The Coomassie stain disappeared after soaking the
PVDF-membranes in 100% methanol prior blocking and antibody steps.
However, now I'm using nitrocellulose membranes where the methanol-step is
omitted. Without a methanol-step it seems that the Coomassie stain will
persist on the membrane also during the first blocking step and all the
following incubation steps. Does the Coomassie stain interfere with the
antibody binding or the chemoluminesent detection (I'm using ECL detection
system)? And if yes, is there a possibility to remove the Coomassie stain
from the NC-membrane before immunoblotting? Or is it only possible to use
Coomassie stained gels directly for immunoblotting only together with
PVDF-membranes?



I would be very grateful for some helpful informations.



Sincerely yours



Catrin Kaydamov

Reply With Quote
Reply

Tags
blot , coomassie , gel , sdspage , stained , western


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Western Blot Background Troubleshooting molecule2005 Western Blot Forum 18 10-07-2010 11:49 AM
Can we use dot blot inplace of Western blot? peterish Western Blot Forum 3 06-16-2010 07:59 PM
Western blot admin Article Discussion 2 04-23-2010 07:31 PM
dot blot and western blot Liang Western Blot Forum 1 08-07-2009 03:26 PM
western blot question Jayakumar, R Protocols and Methods Forum 0 09-27-2007 06:49 PM


All times are GMT. The time now is 07:45 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11084 seconds with 16 queries