why I can see my band dyed in proceau S on PDFC?

Since your technique works in for NC then obviously something is wrong
with your PVDF. Have you hydrated your PVDF in methanol before transfer?
This might a source of the problem

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Today's Topics:

1. why I can see my band dyed in proceau S on PDFC?
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Message: 1
Date: Sat, 25 Nov 2006 10:57:51 +0800
From: "=?GB2312?B?veDV1A==?=" <[Only registered users see links. ]>
Subject: [Protein-analysis] why I can see my band dyed in proceau S on
PDFC?
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sir/madam:
I have been trying to blot proteins of brain from an 8% SDS PAGE
toBioRad PVDF membrane. I thought that my blot was unsuccessful
afterattempting to visualize by Ponceau S. I could not even detect my
MWstandards.but the same process worked well in NCmembrane,where the
band
showed clearly.. Any clues as to why my ponceau S did not work? I
diluted a
fresh Sigam concentrate (20 mL) into 180 mL ofdeionized water. I stained
for
5 min and watch as the membrane destained in 1% AcOH. At no time did I
see
anything resembling a protein band. This problem has occured for the
past
two consecutive times that I have tried the technique. Your comments are
appreciated.


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