Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protein Science > Protein Forum
Register Search Today's Posts Mark Forums Read

Protein Forum Protein Forum


why I can see my band dyed in proceau S on PDFC?

why I can see my band dyed in proceau S on PDFC? - Protein Forum

why I can see my band dyed in proceau S on PDFC? - Protein Forum


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 11-27-2006, 03:46 PM
Tsalavos Sotiris
Guest
 
Posts: n/a
Default why I can see my band dyed in proceau S on PDFC?





Since your technique works in for NC then obviously something is wrong
with your PVDF. Have you hydrated your PVDF in methanol before transfer?
This might a source of the problem

-----Original Message-----
From: [Only registered users see links. ]
[mailtoroteins-bounces@oat.bio.indiana.edu] On Behalf Of
[Only registered users see links. ]
Sent: Saturday, November 25, 2006 7:01 PM
To: [Only registered users see links. ]
Subject: Proteins Digest, Vol 18, Issue 13

Send Proteins mailing list submissions to
[Only registered users see links. ]

To subscribe or unsubscribe via the World Wide Web, visit
[Only registered users see links. ]
or, via email, send a message with subject or body 'help' to
[Only registered users see links. ]

You can reach the person managing the list at
[Only registered users see links. ]

When replying, please edit your Subject line so it is more specific
than "Re: Contents of Proteins digest..."


Today's Topics:

1. why I can see my band dyed in proceau S on PDFC?
(=?GB2312?B?veDV1A==?=)


----------------------------------------------------------------------

Message: 1
Date: Sat, 25 Nov 2006 10:57:51 +0800
From: "=?GB2312?B?veDV1A==?=" <[Only registered users see links. ]>
Subject: [Protein-analysis] why I can see my band dyed in proceau S on
PDFC?
To: [Only registered users see links. ]
Message-ID:
<4d0022c90611241857v48f5c87dj34e2c08f2df449ac@mail .gmail.com>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed

sir/madam:
I have been trying to blot proteins of brain from an 8% SDS PAGE
toBioRad PVDF membrane. I thought that my blot was unsuccessful
afterattempting to visualize by Ponceau S. I could not even detect my
MWstandards.but the same process worked well in NCmembrane,where the
band
showed clearly.. Any clues as to why my ponceau S did not work? I
diluted a
fresh Sigam concentrate (20 mL) into 180 mL ofdeionized water. I stained
for
5 min and watch as the membrane destained in 1% AcOH. At no time did I
see
anything resembling a protein band. This problem has occured for the
past
two consecutive times that I have tried the technique. Your comments are
appreciated.


------------------------------

_______________________________________________
Proteins mailing list
[Only registered users see links. ]
[Only registered users see links. ]

End of Proteins Digest, Vol 18, Issue 13
****************************************

Reply With Quote
Reply

Tags
band , dyed , pdfc , proceau


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Re:why I can see my band dyed in proceau S on PDFC? Lu Falong Protein Forum 2 12-10-2006 08:36 AM
why I can see my band dyed in proceau S on PDFC? Protein Forum 0 11-25-2006 02:57 AM


All times are GMT. The time now is 12:38 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11477 seconds with 16 queries