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why I can see my band dyed in proceau S on PDFC?

why I can see my band dyed in proceau S on PDFC? - Protein Forum

why I can see my band dyed in proceau S on PDFC? - Protein Forum


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Old 11-25-2006, 02:57 AM
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Default why I can see my band dyed in proceau S on PDFC?



sir/madam:
I have been trying to blot proteins of brain from an 8% SDS PAGE
toBioRad PVDF membrane. I thought that my blot was unsuccessful
afterattempting to visualize by Ponceau S. I could not even detect my
MWstandards.but the same process worked well in NCmembrane,where the band
showed clearly.. Any clues as to why my ponceau S did not work? I diluted a
fresh Sigam concentrate (20 mL) into 180 mL ofdeionized water. I stained for
5 min and watch as the membrane destained in 1% AcOH. At no time did I see
anything resembling a protein band. This problem has occured for the past
two consecutive times that I have tried the technique. Your comments are
appreciated.
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Re:why I can see my band dyed in proceau S on PDFC? Lu Falong Protein Forum 2 12-10-2006 08:36 AM
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