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[Protein-analysis] Re: protein shape change detection

[Protein-analysis] Re: protein shape change detection - Protein Forum

[Protein-analysis] Re: protein shape change detection - Protein Forum


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Old 09-07-2006, 05:31 PM
Carlo Zambonelli
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Default [Protein-analysis] Re: protein shape change detection



>

CD and fluorescence give you information about secondary structure, not
shape. I really mean shape: oval, globular, rod-like, straight, bent, etc..
No unfolding involved in transition between different "shapes" and probably
little 2nd structure modification.
No, crystallography or EM are not viable options.
Carlo
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Old 09-22-2006, 01:54 PM
Dr Engelbert Buxbaum
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Default [Protein-analysis] Re: protein shape change detection

Carlo Zambonelli wrote:


In that case, information on shape will be incomplete, but you may be
able to get coarse figures like for example the ratio of the eliptical
axes of a protein. In as far as conformational changes affect the
molecular hydrodynamics of a protein small angle neutron or light
scattering, analytical ultracentrifugation, gel filtration or native
electrophoresis can follow them. Full interpretation however will not be
possible without more detailed data on the endpoint-structures.
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