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Protein refolding (uhh...) by rapid dilution

Protein refolding (uhh...) by rapid dilution - Protein Forum

Protein refolding (uhh...) by rapid dilution - Protein Forum


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Old 09-07-2006, 06:16 PM
Julio Cortazar
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Default Protein refolding (uhh...) by rapid dilution



Hi everybody,

do u have exprience in this technique? I'm planning the experiments (as
usual sets of conditions etc) Tell me, if u will be so kind, is it
better to make renaturation eg shorter but 20Cdeg, or inice cold
reneturation buffer for longer time? Have u heard about saccharose,
that it stabilise denatured-native intermendiates?

I would appreciate all news

Breslauer (what mean citizen of Breslau

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dilution , protein , rapid , refolding , uhh


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