I'm trying to find out what is the best technique to detect protein shape
change (i.e. globular to non-globular) in solution. I believe the only
options are analytical ultracentrifugation or SEC-MALLS. I don't have
experience with either technique and I would appreciate any feedback,
Thank you very much.
Have you considered using the standard biophysical techniques like CD
and fluorescence? It sounds very much like you are doing something
similar to a protein unfolding experiment, and many of the techniques
that are used to study folding should be suitable for what you want.
In article <[Only registered users see links. ].net >,
"Carlo Zambonelli" <[Only registered users see links. ]> wrote:
It would be best to find a collaborator who is expert in the area or
plan on spending a lot of time (the analysis is relatively simple but
since it involves model fitting it needs very good data and obtaining a
good data set will take a lot of time and effort). The developer of the
ULTRASCAN software package Bories Demeler at UT San Antonio has a couple
of 2-3 day courses on the software package etc. that is offered once or
twice a year. The other developers (Peter Schuck) offer similar courses
a few times a year though sometimes they are targeted at more advanced
users. If you cannot make it for one of these courses contacting them
may put you in contact with a suitable euro labs.