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Carlo Zambonelli 09-05-2006 12:59 AM

protein shape change detection
 
Hi,
I'm trying to find out what is the best technique to detect protein shape
change (i.e. globular to non-globular) in solution. I believe the only
options are analytical ultracentrifugation or SEC-MALLS. I don't have
experience with either technique and I would appreciate any feedback,
reference, etc.
Thank you very much.
CZ

Geoff Briggs 09-06-2006 07:59 AM

protein shape change detection
 
Carlo Zambonelli wrote:


Have you considered using the standard biophysical techniques like CD
and fluorescence? It sounds very much like you are doing something
similar to a protein unfolding experiment, and many of the techniques
that are used to study folding should be suitable for what you want.

Geoff

Joe Doe 09-07-2006 11:35 PM

protein shape change detection
 
In article <[Only registered and activated users can see links. Click Here To Register...].net >,
"Carlo Zambonelli" <[Only registered and activated users can see links. Click Here To Register...]> wrote:



It would be best to find a collaborator who is expert in the area or
plan on spending a lot of time (the analysis is relatively simple but
since it involves model fitting it needs very good data and obtaining a
good data set will take a lot of time and effort). The developer of the
ULTRASCAN software package Bories Demeler at UT San Antonio has a couple
of 2-3 day courses on the software package etc. that is offered once or
twice a year. The other developers (Peter Schuck) offer similar courses
a few times a year though sometimes they are targeted at more advanced
users. If you cannot make it for one of these courses contacting them
may put you in contact with a suitable euro labs.

Roland


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