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Enzyme assay

Enzyme assay - Protein Forum

Enzyme assay - Protein Forum

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Old 08-15-2006, 07:55 AM
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Default Enzyme assay

Dear all, I want to perform an enzyme inhibition assay, but I'm not
sure about the concentration of substrate I would beter use. is it
better to use conc. equal to Km value, or less than it? what if I'll
use fluorimetric assay of the product?

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