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[Protein-analysis] pure protein (?) but two bands onSDS-PAGE

[Protein-analysis] pure protein (?) but two bands onSDS-PAGE - Protein Forum

[Protein-analysis] pure protein (?) but two bands onSDS-PAGE - Protein Forum


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Old 07-28-2006, 08:11 AM
Rhiannon Evans
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Default [Protein-analysis] pure protein (?) but two bands onSDS-PAGE



Thanks, but I dont think there are any glycosylation pathways in E.coli and I
have also tried de-phosphorylation but it makes no difference. Are there any
other common post-translational modifications to consider?

Some apparently pure proteins often run as doublets - this could represent
slightly different glycosylation forms, or other post-translational
modifications, or may represent protein-protein interactions/dimerisation.
Its not uncommon.

On 7/25/06, Rhiannon Evans <[Only registered users see links. ].uk> wrote:



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