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trypsin autolysis

trypsin autolysis - Protein Forum

trypsin autolysis - Protein Forum


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  #1  
Old 05-23-2006, 12:11 PM
Ann
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Default trypsin autolysis



hello folks,

Can someone tell me how i can prevent my purified trypsin from
autolysis...

I have tried CaCl2 but it has not given me good results.

Ann

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  #2  
Old 06-01-2006, 08:08 PM
byron
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Default trypsin autolysis

What kind of trypsin are you using? We use Promega's Mass Spec grade
modified trypsin, and it does give some autolysis, but not enough to
interfere with protein ID.

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