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-   -   trypsin autolysis (http://www.molecularstation.com/forum/protein-forum/26712-trypsin-autolysis.html)

Ann 05-23-2006 12:11 PM

trypsin autolysis
 
hello folks,

Can someone tell me how i can prevent my purified trypsin from
autolysis...

I have tried CaCl2 but it has not given me good results.

Ann


byron 06-01-2006 08:08 PM

trypsin autolysis
 
What kind of trypsin are you using? We use Promega's Mass Spec grade
modified trypsin, and it does give some autolysis, but not enough to
interfere with protein ID.



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