Any Blue native gel expert?

Hi there,

Wonder if somebody with blue native gel expertise and
experience can help me with some advice. We are trying to
do blotting of the 1st dimension of BN-PAGE and so far are
completely baffled since the transfer process seemed not to
work, as revealed as no bands observed upon western
procedures. I read in one of the previous messages some
people mentioned about the possibility of the target protein
precipitating in the gel and therefore doesn't want to move
to the membrane but how would we test for that, and if it
does precipitate, how to overcome the problem??

Would appreciate very much any inputs.

Thanks,
Melani
Melani Atmodjo
Ph.D Student
Dept. of Biochemistry & Molecular Biology
University of Georgia
Room #2047, Complex Carbohydrate Research Center
315 Riverbend Road, Athens, GA 30602
(706) 542-4499 (T)