Prestained SDS-PAGE markers WITHOUT reducing agent

Dear all,
I am trying to find a company that sells prestained
SDS-PAGE markers without added reducing agent. All of
the vendors I have found included reducing agents in
their products.
Can someone point me in the right direction?
Thank you very much.
D. Andrews
University of Miami

--- Dr Engelbert Buxbaum
<[Only registered users see links. ]> wrote:



__________________________________________________
Do You Yahoo!?
Tired of spam? Yahoo! Mail has the best spam protection around
[Only registered users see links. ]

In article <[Only registered users see links. ].net >, DA <[Only registered users see links. ]> wrote:

Buy whatever prestained markers you like and run
them over little spin gel-filtration columns.
(Bunch of companies sell them and they are trivial to
make out of blue tip, for example). Volume change
is minimal and the protein recovery is always >80%.

Sephadex G-25 is most common but I like
Bio-Rad's P6 better.

DK

If you leave a lane free between the markers and your non-reduced
samples you don't need to remove the reducing agent. I have done this
many times.
Allison

DK wrote:

Yes, this is true, but sometimes, depending on the composition of the
markers, the non-reduced forms can run differently to the reduced forms, so
for accurate MW measurement you need to be sure that there is no difference
before resorting to this method - at least, that's been my experience...

Amanda


On 3/18/06, Allison <[Only registered users see links. ]> wrote:
If at first you don't succeed, destroy all evidence that you tried --- Homer
Simpson

I use BioRad's kaleidoscope markers on my non-reduced zymograms and reverse
zymograms all the time (testing for gelatinase activity in my samples) and
they have never given me any problems - I don't even have to leave a lane
between the marker and the sample - they don't seem to leak any kind of
interfering reducing agent.

I know this probably doesn't answer your question, but if you're looking for
markers to use with non-reduced samples, that's what I use without any
problems...

Amanda Gillespie
Postgraduate student - Biochemistry and Immunology
UKZN
South Africa

On 3/20/06, DA <[Only registered users see links. ]> wrote:



--
If at first you don't succeed, destroy all evidence that you tried --- Homer
Simpson