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[Protein-analysis] Re: DLS at high protein concentration

[Protein-analysis] Re: DLS at high protein concentration - Protein Forum

[Protein-analysis] Re: DLS at high protein concentration - Protein Forum


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Old 03-15-2006, 12:52 PM
QCZ
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Default [Protein-analysis] Re: DLS at high protein concentration



Zhao,
Thanks for your input. Sorry I did not indicate that I was using a
batch-mode ALV/CGS-3 machine and it has an attenuator. So the flow rate and
signal saturation are not problems for me in this case. I believe that what
is measured is D(app). D(app)=D(0)*(1+k*c). So extrapolation to c=0 would
give us the intrinsic diffusion coefficient that can be used to calculate
the intrinsic Rh.

Regards,

Chin
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