Candida proteomics

Dear all,

I want to extract proteins from Candida biofilms grown in 1cmm acrylic coupons.
Obtaining cell pellet is pretty small although I pool few coupons together.

Can you please tell me a protocol to extract proteins from this. Here I am
looking for extrac-cellular proteins as well as Candida cellular proteins.

I tried with glassbeads, it didn't work.

Please help,

C.J.Seneviratne,
University of Hong Kong.

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I have never done that with Candida - but for S. cerevisiae the glass
bead method worked well for me. A few things to consider: Make sure you
have the right size beads - if they are too large grinding doesn't work.
You have to vortex *vigorously* and long enough. Try a few different
lysis buffers - I had good results with an SDS/urea buffer. Maybe try to
use more cells.

Clontech has a pretty good protocols handbook online - it's for S.
cerevisiae but I think most of the recipes should work just as well for
Candida:

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hope it helps

Philipp


--
Dr. Philipp Pagel Tel. +49-8161-71 2131
Dept. of Genome Oriented Bioinformatics Fax. +49-8161-71 2186
Technical University of Munich
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