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Bacterial contamination of yeast two hybrid

Bacterial contamination of yeast two hybrid - Protein Forum

Bacterial contamination of yeast two hybrid - Protein Forum


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  #1  
Old 03-09-2006, 12:44 PM
S.Ananth@liverpool.ac.uk
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Default Bacterial contamination of yeast two hybrid



Hi,
I am a final year PhD student at the University of Liverpool.
I have been carrying out yeats two hybrid experiments as a part of project work.
I seem to be facing the problem of bacterial contamination. In this regard I
read a message in google by Schmidt Thorsten. I was searching for the reply to
the message but couldnt access it.
I seem to have a similar problem of bacterial contamination in my yeast culture
and wanted to know if there are any possibilties of eliminating it by the use
of a suitable antibiotic and also the concentration. Also could the use of the
antibiotic affect the growth of the yeast.

It would be great help if you could kindly suggest something to sort this
problem.


Cheers
Sahana

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  #2  
Old 03-10-2006, 04:41 AM
DK
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Default Bacterial contamination of yeast two hybrid

In article <[Only registered users see links. ].net> , [Only registered users see links. ] wrote:

100 years ago people were obtaining pure cultures by serial
plating/streaking of cultures and selecting colonies of interest
without any antibiotics. There is no reason why you cannot do
it now. The difference between yeast and bacterial colonies
is huge, so it should be easy.

DK

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  #3  
Old 05-28-2011, 03:40 AM
Pipette Filler
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Default Re: Bacterial contamination of yeast two hybrid

I believe that most yeast can survive 50 ug/ml Kanamycin, whereas E.coli can not.
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