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Ni-NTA regeneration

Ni-NTA regeneration - Protein Forum

Ni-NTA regeneration - Protein Forum


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  #1  
Old 01-12-2006, 10:53 AM
Julio
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Default Ni-NTA regeneration



Hi there, Has anybody tried to regenerate Qiagen Ni-NTA resign using
Ni(NO3)2 instead NiSO4? Dores it make a difference???

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  #2  
Old 01-12-2006, 11:00 PM
D.K.
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Default Ni-NTA regeneration

In article <1137063220.965081.96160@g49g2000cwa.googlegroups. com>, "Julio" <[Only registered users see links. ]> wrote:

It shouldn't. I never use nitrate but used chloride and it made
no difference whatsoever.

DK

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  #3  
Old 01-14-2006, 03:45 PM
Julio
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Default Ni-NTA regeneration

Yes, u r right! If I can use NaCl in buffers so NiCl2 should be inert
for the resign.

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