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trypsin digestion

trypsin digestion - Protein Forum

trypsin digestion - Protein Forum


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  #1  
Old 12-17-2005, 04:15 AM
nithya
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Default trypsin digestion



I have done a trypsin digestion of a coloured protein.........
my protocol is 1mg/ml trypsin in 0.1% acetic acid.......
protein soln 40 ug/ml in 100 mM AmBic buffer ph:8.5
overnight incubation
prot: trypsin ratio : 100:1/ 50:1

my boss says that i need to c a reduction in color of the prot if
digestion has occurred.....

is ther any other way to confirm that digestion has occurred
completely?

I would welcome any suggestions on the protocol as well

have to do MS of the digested peptides

help me

thanks

Nithya

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  #2  
Old 12-17-2005, 06:07 PM
Kyle Legate
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Default trypsin digestion

nithya wrote:
Run an SDS-PAGE gel--does your full-length protein disappear? You'll
have to run a gel before MS anyway.
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