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07-17-2005 09:11 PM

Bradford Protein Assay in membrane crystals
 
Another alternative: get rid of lipids while keeping the proteins dissolved:
extract your goo with butanol (20%v/v,a standard method for membrane protein
purification).
The supernatant n-butanol will have most of the lipids. The
butanol-saturated water phase is good for Bradford.

Lajos


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