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Bradford Protein Assay in membrane crystals Another alternative: get rid of lipids while keeping the proteins dissolved: extract your goo with butanol (20%v/v,a standard method for membrane protein purification). The supernatant n-butanol will have most of the lipids. The butanol-saturated water phase is good for Bradford. Lajos will "Gerd Hoffmann" <[Only registered and activated users can see links. Click Here To Register...]> wrote in message news:[Only registered and activated users can see links. Click Here To Register...]... |
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