Bradford Protein Assay in membrane crystals
Another alternative: get rid of lipids while keeping the proteins dissolved:
extract your goo with butanol (20%v/v,a standard method for membrane protein
The supernatant n-butanol will have most of the lipids. The
butanol-saturated water phase is good for Bradford.
will "Gerd Hoffmann" <[Only registered and activated users can see links. Click Here To Register...]> wrote in message
news:[Only registered and activated users can see links. Click Here To Register...]...
|All times are GMT. The time now is 11:12 AM.|
Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved