I would like to try to extract a biotin-tagged voltage-gated ion
channels from transfected HEK cells (alternatively I could use C6 or
astrocytes, but I prefer HEKs). I have no experience in biochemistry, I
apologize for oversimplifying the problem.
I have streptavidin DYnabeads that I can use to pull off the
biotin-tagged protein. I've never done this before, does anyone have
any suggestions or potential problems.
Also, what would be the best way to find out whether I have enough
protein to see it on a gel? This is perhaps my biggest concern, I'd
like to be sure that there is enough sample.