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how to isolate surface membrane proteins?

how to isolate surface membrane proteins? - Protein Forum

how to isolate surface membrane proteins? - Protein Forum


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Old 05-15-2004, 09:01 AM
brenda
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Default how to isolate surface membrane proteins?



Hi there,

For my project I need to make clean Westren blots. The only way I have not
tried yet is to purify the membrane fraction from my cells (BAF/B03, which
is a murine precursor B cell line). The protein I am looking for is the
human growth hormone receptor, which is a membrane bound protein.
Can somebody please tell me how to isolate this membrane fraction? I have
heard about specialised kits (but without references) and some techniques
using high speed centrifugation.
Please help me out.

Thanks a lot,
Brenda


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