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co-ip of bacteria membrane protein

co-ip of bacteria membrane protein - Protein Forum

co-ip of bacteria membrane protein - Protein Forum


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  #1  
Old 11-02-2003, 08:08 PM
mj
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Default co-ip of bacteria membrane protein



Hi, all

I am planning to do a co-IP on an inner membrane protein with HA tag of
Caulobacter(a gram negative bacterium), and I am hoping to pull down some
protein complexes under non-denaturing condition (low detergent). Has
anybody done similar experiments like this? I really don't know where to
start, does anybody have a protocol for that?

How shall I determine the right condition that can solubilize the membrane
protein yet maintain the complexes? For HA tag, which antibody will be
better, monoclonal or polyclonal?

Thank you very much! Suggestion will be really appreciated!



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Old 11-04-2003, 05:33 AM
Dr Engelbert Buxbaum
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Default co-ip of bacteria membrane protein

mj wrote:


Calbiochem offers heat-killed protein A (Pansorbin) or protein G
containing bacteria for immunoprecipitation experiments, and they have
published a little booklet on methods. You may wish to consult this.

Usual disclaimer applies.
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