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mj 11-02-2003 08:08 PM

co-ip of bacteria membrane protein
 
Hi, all

I am planning to do a co-IP on an inner membrane protein with HA tag of
Caulobacter(a gram negative bacterium), and I am hoping to pull down some
protein complexes under non-denaturing condition (low detergent). Has
anybody done similar experiments like this? I really don't know where to
start, does anybody have a protocol for that?

How shall I determine the right condition that can solubilize the membrane
protein yet maintain the complexes? For HA tag, which antibody will be
better, monoclonal or polyclonal?

Thank you very much! Suggestion will be really appreciated!




Dr Engelbert Buxbaum 11-04-2003 05:33 AM

co-ip of bacteria membrane protein
 
mj wrote:


Calbiochem offers heat-killed protein A (Pansorbin) or protein G
containing bacteria for immunoprecipitation experiments, and they have
published a little booklet on methods. You may wish to consult this.

Usual disclaimer applies.


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