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-   -   protein folding on Ni-column (http://www.molecularstation.com/forum/protein-forum/26010-protein-folding-ni-column.html)

Schwabe, TME 09-11-2003 05:59 PM

protein folding on Ni-column
 
Wow, this might be my chance! Yes, there is a reference I happen to
remember:
Schwabe, T. M., Gloddek, K., Schluesener, D. and Kruip, J., Purification
of recombinant BtpA and Ycf3, proteins involved in membrane protein
biogenesis in Synechocystis PCC 6803. J Chromatogr B Analyt Technol
Biomed Life Sci, 2003. 786(1-2): p. 45-59

:-)

Actually we did it the way DK suggested, by a gradient from 6M to 0M
urea at RT. It was easy for the first protein and gave very good
results, protein #2 didn't like it, but this one was more hydrophobic...

There is also an application manual from Amersham (should belong to
their ÄKTA series), which has a procedure about this.


Tatjana

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