protein folding on Ni-column
Wow, this might be my chance! Yes, there is a reference I happen to
Schwabe, T. M., Gloddek, K., Schluesener, D. and Kruip, J., Purification
of recombinant BtpA and Ycf3, proteins involved in membrane protein
biogenesis in Synechocystis PCC 6803. J Chromatogr B Analyt Technol
Biomed Life Sci, 2003. 786(1-2): p. 45-59
Actually we did it the way DK suggested, by a gradient from 6M to 0M
urea at RT. It was easy for the first protein and gave very good
results, protein #2 didn't like it, but this one was more hydrophobic...
There is also an application manual from Amersham (should belong to
their ÄKTA series), which has a procedure about this.
[Only registered and activated users can see links. Click Here To Register...] wrote:
|All times are GMT. The time now is 01:28 AM.|
Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved