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protein folding on Ni-column

protein folding on Ni-column - Protein Forum

protein folding on Ni-column - Protein Forum


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Old 09-04-2003, 08:01 PM
D.K.
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Default protein folding on Ni-column



In article <[Only registered users see links. ]>, [Only registered users see links. ] wrote:

No refs that I have on hand but depending on the protein, one of the
two extreme approaches should work:
1. Quickly dump and mix your sepharose-bound protein into
100-300 column volumes of a solution without denaturant and let
sit for an hour or so.
2. Run slow (20-30 column volumes) gradient from 100% to 0%
denaturant through a column.

Both done better at RT but there are, I believe, some examples
of #2 working better in the cold room.

DK

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