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one pure protein but has two clear bands in SDS-PAGE

one pure protein but has two clear bands in SDS-PAGE - Protein Forum

one pure protein but has two clear bands in SDS-PAGE - Protein Forum


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Old 07-29-2003, 06:52 AM
chengzhjun@hotmail.com
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Default one pure protein but has two clear bands in SDS-PAGE



We have got several proteins here encountering the same problems! Our recombinant protein is on Pet22-b Vector and expressed in Bl21(DE3) E.coli strain. After purification by NTA-Ni column, we see two clear bands in the SDS-PAGE. The two bands have a similar concentration and they are very near to each other. In our protein solutions ,we have already added some protease inhibitor.
Anybody can help me to explain this phenomenon? Any suggestion is highly appreciated.
By the way,how to attach photos? I want to attach my SDS-PAGE result so that you can get a direct understanding of my problems.


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Old 07-29-2003, 08:20 AM
Duncan Clark
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Default one pure protein but has two clear bands in SDS-PAGE

Historians believe that in newspost
<[Only registered users see links. ]> on Tue, 29 Jul 2003,
[Only registered users see links. ] penned the following literary masterpiece:

You can't/shouldn't - it is a newsgroup and a non-binary one at that.
Put them on a website and paste a URL.

Duncan
--
I love deadlines. I especially like the whooshing noise they make as
they go flying by.

Duncan Clark
GeneSys Ltd.
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