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problem in protein expression

problem in protein expression - Protein Forum

problem in protein expression - Protein Forum


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  #1  
Old 06-30-2003, 04:46 PM
EK
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Default problem in protein expression




"SCK" <[Only registered users see links. ]> wrote in message
news:bdplir$e5e$[Only registered users see links. ].cuhk.edu.hk...
coli.
expected
No stop codon + no transcription terminator ?
- EK


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Old 07-03-2003, 10:16 AM
Duncan Clark
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Default problem in protein expression

Historians believe that in newspost
<bduluf$2lpo$[Only registered users see links. ].cuhk.edu.hk> on Wed, 2 Jul 2003, SCK
<[Only registered users see links. ]> penned the following literary masterpiece:

If you are sure your SDS gel really is denaturing then you have either
cloned a larger ORF than you think or you don't have a stop codon and
are cloned in frame with plasmid DNA so generate a fusion or you have
protein that runs at an anomalous mwt from that predicted. Sequencing is
the key.

Duncan
--
I love deadlines. I especially like the whooshing noise they make as
they go flying by.

Duncan Clark
GeneSys Ltd.
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