Lowry protein determination
 

"Kyle Legate" <[Only registered and activated users can see links. Click Here To Register...]> wrote in message
news:apjKa.4884$[Only registered and activated users can see links. Click Here To Register...].. .
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0.248 sounds like a blank reading of OD595 for me. Subtract that value from
all samples if you did not do that. If you do that and get the intersection
at 0.248, try producing another standard curve with lower concentrations of
BSA in addition to ones you have used in your previous experiment. Se how
the curve goes. It might be that your are going over the linear range of
detection with concentrations you have used. Check with the provider of your
Coomassie reagent on the detection range (or read it the manual:-)). Also,
don't use 1x Coomassie mixture that is older that 2 weeks.
Emir