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Protein detection problem under reducing condition

Protein detection problem under reducing condition - Protein Forum

Protein detection problem under reducing condition - Protein Forum


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Old 06-25-2003, 11:46 AM
Mauro Bongrazio
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Default Protein detection problem under reducing condition



Hallo everybody,
I have a home-made anti-peptide antibody which detects the target
protein under non-reducing but not under reducing (beta-mercapto)
conditions. Do you know why? I thought the contrary could be the
case…Thanks
Mauro

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Old 06-25-2003, 01:27 PM
EK
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Default Protein detection problem under reducing condition


""Mauro Bongrazio"" <[Only registered users see links. ]-berlin.de> wrote in message
news:[Only registered users see links. ]-Berlin.DE...
Hallo everybody,
I have a home-made anti-peptide antibody which detects the target
protein under non-reducing but not under reducing (beta-mercapto)
conditions. Do you know why? I thought the contrary could be the
case.Thanks
Mauro

---

Obviously, you are unfolding the antibody and thus disrupting the
recognition surface. This is very common. Now you know that your antibody
has a surface, rather than a linear sequence, that recognizes your peptide.
BME reduces disulfide bonds that in your case contribute to correct folding
of the Ab.
-Emir


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