How to avoid denatured protein?

[Xerophytes]

Just wondering how do you actually avoid denatured protein? I got a protein that almost always result into a precipitation when I carry out my crystallization. I read that it is actually denatured protein and this will never crystallize, is there a way to get around this?

[sharonbaker]

Hello friend,

I am also finding the same.... Hope someone knowledgeable come over this forum site and try to introduce about "How to avoid denatured protein"... Thanks in advance...

[vakkomak]

hi,

Take care of the temperature when dealing with the protein. Most importantly, check the pH of the solution conditions under which your protein is present.
Another thing to keep in mind, is the amount of protein you are using in your crystallization experiments. the optimum concentration is 10 mg/ml. I normally use 5 mg/ml.